首页> 外文OA文献 >Innate immune detection of the type III secretion apparatus through the NLRC4 inflammasome
【2h】

Innate immune detection of the type III secretion apparatus through the NLRC4 inflammasome

机译:通过NLRC4炎性小体对III型分泌设备的先天免疫检测

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

The mammalian innate immune system uses Toll-like receptors (TLRs) and Nod-LRRs (NLRs) to detect microbial components during infection. Often these molecules work in concert; for example, the TLRs can stimulate the production of the proforms of the cytokines IL-1β and IL-18, whereas certain NLRs trigger their subsequent proteolytic processing via caspase 1. Gram-negative bacteria use type III secretion systems (T3SS) to deliver virulence factors to the cytosol of host cells, where they modulate cell physiology to favor the pathogen. We show here that NLRC4/Ipaf detects the basal body rod component of the T3SS apparatus (rod protein) from S. typhimurium (PrgJ), Burkholderia pseudomallei (BsaK), Escherichia coli (EprJ and EscI), Shigella flexneri (MxiI), and Pseudomonas aeruginosa (PscI). These rod proteins share a sequence motif that is essential for detection by NLRC4; a similar motif is found in flagellin that is also detected by NLRC4. S. typhimurium has two T3SS: Salmonella pathogenicity island-1 (SPI1), which encodes the rod protein PrgJ, and SPI2, which encodes the rod protein SsaI. Although PrgJ is detected by NLRC4, SsaI is not, and this evasion is required for virulence in mice. The detection of a conserved component of the T3SS apparatus enables innate immune responses to virulent bacteria through a single pathway, a strategy that is divergent from that used by plants in which multiple NB-LRR proteins are used to detect T3SS effectors or their effects on cells. Furthermore, the specific detection of the virulence machinery permits the discrimination between pathogenic and nonpathogenic bacteria.
机译:哺乳动物的先天免疫系统使用Toll样受体(TLR)和Nod-LRR(NLR)来检测感染期间的微生物成分。这些分子经常协同工作。例如,TL​​R可以刺激细胞因子IL-1β和IL-18的形式产生,而某些NLR通过caspase 1触发其随后的蛋白水解过程。革兰氏阴性细菌使用III型分泌系统(T3SS)传递毒力。是宿主细胞胞质溶胶的主要因子,在那里它们调节细胞生理以促进病原体生长。我们在这里显示NLRC4 / Ipaf从鼠伤寒沙门氏菌(PrgJ),假伯克霍尔德氏菌(BsaK),大肠杆菌(EprJ和EscI),弗氏志贺氏菌(MxiI)和铜绿假单胞菌(PscI)。这些杆蛋白共有一个序列基序,这对于NLRC4检测至关重要。在鞭毛蛋白中发现了类似的基序,也被NLRC4检测到。鼠伤寒沙门氏菌有两个T3SS:沙门氏菌致病岛-1(SPI1),它编码杆蛋白PrgJ,和SPI2,其编码杆蛋白SsaI。尽管NLRC4可检测到PrgJ,但SsaI却未检测到,而这种逃避是小鼠毒性的必需条件。检测T3SS装置的保守成分可以通过单一途径对毒性细菌进行先天免疫反应,这种策略与植物使用的策略不同,在植物中,多个NB-LRR蛋白用于检测T3SS效应子或其对细胞的影响。此外,对毒力机制的特异性检测可以区分致病菌和非致病菌。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号